数据文件配置说明
安装数据文件，配置说明！
1、下载database.zip文件，
压缩包的目录结构为：
2、在软件安装目录解压缩database.zip文件。确保 VGenomics_RS/database/diamond_db 目录下存在以下4个文件：
测试数据详细信息！
测试数据：ref-based_test_rawdata.zip，
压缩包，包含文件列表如下所示：
数据说明：
| chr22_with_ERCC92.fa | only a single chromosome (chr22) and the ERCC spike-in, that is the human GRCh38 version of the genome from Ensembl. |
| chr22_with_ERCC92.gtf | annotations obtained from Ensembl (Homo_sapiens.GRCh38.86.gtf.gz) for chromosome 22 only. |
| gene_GO_anno.txt | GO functional annotation file. |
| gene_KEGG_anno.txt | KEGG functional annotation file. |
| *.fastq | The test data consists of two commercially available RNA samples: Universal Human Reference (UHR)and Human Brain Reference (HBR) . The UHR is total RNA isolated from a diverse set of 10 cancer cell lines. The HBR is total RNA isolated from the brains of 23 Caucasians, male and female, of varying age but mostly 60-80 years old. In addition, a spike-in control was used. Specifically we added an aliquot of the ERCC ExFold RNA Spike-In Control Mixes to each sample. The spike-in consists of 92 transcripts that are present in known concentrations across a wide abundance range (from very few copies to many copies). |
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