Single sample Taxonomy analysis tree(单样品分类学系统组成树)
根据每个样本或者多个样本与Silva或者RDP数据库的分类学比对结果,选出优势物种(丰度前N或所占百分比大于指定p)的分类,以此同时画出其所包含在的门纲目科,以树枝状呈现。
单个样本图中圆圈大小代表该物种在该水平所占的比重,圆圈下的数字,第一个表示只比对到该物种(不能比对到该级以下的水平)的序列数,第二个数字表示共有多少序列比对到该物种。
输入:
OTU Table文件:
OTU ID Bio1 Bio2 Bio3 Bio4 Bio5 Bio6 Bio7 Bio8 Bio9 Bio10
OTU1 0 0 0 0 0 6 34 104 367 254
OTU2 52 335 18 49 0 0 0 0 0 0
OTU3 0 0 0 0 5 0 0 0 0 0
对应OTU的分类学注释文件:
OTU1 d__Bacteria; p__Bacteroidetes; c__Cytophagia; o__Cytophagales; f__Cytophagaceae; g__Ohtaekwangia; s__uncultured_bacterium
OTU10 d__Bacteria; p__Planctomycetes; c__Phycisphaerae; o__Phycisphaerales; f__Phycisphaeraceae; g__SM1A02; s__uncultured_Planctomycetales_bacterium
OTU100 d__Bacteria; p__Actinobacteria; c__Actinobacteria; o__Micrococcales; f__Micrococcaceae; g__Arthrobacter; s__Arthrobacter_oryzae
OTU1000 d__Bacteria; p__Proteobacteria; c__Alphaproteobacteria; o__Rhodospirillales; f__Rhodospirillaceae; g__Skermanella; s__uncultured_bacterium
OTU1001 d__Bacteria; p__Proteobacteria; c__Deltaproteobacteria; o__Myxococcales; f__Haliangiaceae; g__Haliangium
OTU1002 d__Bacteria; p__Bacteroidetes; c__Flavobacteriia; o__Flavobacteriales; f__Flavobacteriaceae; g__Chryseobacterium
OTU1003 d__Bacteria; p__Proteobacteria; c__Gammaproteobacteria
OTU1004 d__Bacteria; p__Proteobacteria; c__Alphaproteobacteria; o__Rhizobiales; f__JG35-K1-AG5; g__norank; s__uncultured_bacterium
OTU1005 d__Bacteria; p__Proteobacteria
输出:
图. Single sample Taxonomy analysis tree