PBA邻近编码技术

邻近编码技术

突破性的单外泌体检测技术，改变了传统外泌体技术往往局限于总蛋白、总核酸检测的困境，实现了高精度、高灵敏度的外泌体异质性研究。

邻近编码技术（Proximity Barcoding Assay，PBA）文章：Wu et al. (2019) Profiling surface proteins on individual exosomes using a proximity barcoding assay

PBA原理概述

样本中单个外泌体的表面蛋白组成通过与之结合的抗体-DNA偶联物可被转化为DNA序列信息。

流程步骤

1、抗体-DNA偶联物识别外泌体表面蛋白并结合，PBA技术可以实现同时对单外泌体表面200+蛋白进行识别标记。

2、滚环DNA序列通过识别单外泌体表面标记物，实现单外泌体分隔并标记。

3、新一代测序技术解析DNA序列中的蛋白与外泌体信息。

4、生物信息学分析单外泌体蛋白组学信息。

创新与优势

1、高通量单外泌体（10万）分析

对于每个样本中10万+的单外泌体蛋白进行同步分析，为您发现样本中低丰度外泌体亚群。

2、<5ul样品量且无需提纯

绝大部分仅仅需要微量样本，无需预先进行外泌体分离纯化步骤，进一步节约时间与实验成本。

3、单信号分析检测高灵敏度的数据结果，检测分辨率精确至单外泌体表面分子蛋白，可识别具有更高疾病诊断价值的低丰度外泌体表面蛋白。

4、适用于几乎所有体液、培养液

例如血浆、尿液、细胞培养液、器官灌洗液均能进行单外泌体检测，适用样本类型广泛，可以实现更多不同种类样本的单外泌体检测，大大减少了样本的各类限制。

• Wu, Jicheng et al. “Tumor circulome in the liquid biopsies for cancer diagnosis and prognosis.” Theranostics vol. 10,104544-4556. 15 Mar. 2020, doi:10.7150/thno.40532

• Kucuk, Nika et al. “Exosomes Engineering and Their Roles as Therapy Delivery Tools, Therapeutic Targets, and Biomarkers.” International journal of molecular sciences vol. 22,179543. 2 Sep. 2021, doi:10.3390/ijms22179543

• Padmasekar, Manju et al. “Exposomes to Exosomes: Exosomes as Tools to Study Epigenetic Adaptive Mechanisms in High-Altitude Humans.” International journal of environmental research and public health vol. 18,16 8280. 5 Aug. 2021, doi:10.3390/ijerph18168280

• Yang, Dexin et al. “Combined Analysis of Surface Protein Profile and microRNA Expression Profile of Exosomes Derived from Brain Microvascular Endothelial Cells in Early Cerebral Ischemia.” ACS omega vol. 6,34 22410-22421. 18 Aug. 2021, doi:10.1021/acsomega.1c03248

• Pasetto, Laura et al. “Decoding distinctive features of plasma extracellular vesicles in amyotrophic lateral sclerosis.” Molecular neurodegeneration vol. 16,1 52. 10 Aug. 2021, doi:10.1186/s13024-021-00470-3

• Paolillo, Mayra et al. “Fostering "Education": Do Extracellular Vesicles Exploit Their Own Delivery Code?” Cells vol. 10,71741. 9 Jul. 2021, doi:10.3390/cells10071741

• Shan, Xiaoxiao et al. “The Biogenesis, Biological Functions, and Applications of Macrophage-Derived Exosomes.” Frontiers in molecular biosciences vol. 8 715461. 21 Jul. 2021, doi:10.3389/fmolb.2021.715461

• Guo, Wei et al. “Single-Exosome Profiling Identifies ITGB3+ and ITGAM+ Exosome Subpopulations as Promising Early Diagnostic Biomarkers and Therapeutic Targets for Colorectal Cancer.” Research (Washington, D.C.) vol. 6 (2023): 0041. doi:10.34133/research.0041

• Sun, Li, and David G Meckes Jr. “Multiplex protein profiling method for extracellular vesicle protein detection.” Scientific reports vol. 11,1 12477. 14 Jun. 2021, doi:10.1038/s41598-021-92012-6

• Mitchell, Megan I et al. “Extracellular Vesicle Capture by AnTibody of CHoice and Enzymatic Release (EV-CATCHER): A customizable purification assay designed for small-RNA biomarker identification and evaluation of circulating small-EVs.” Journal of extracellular vesicles vol. 10,8 (2021): e12110. doi:10.1002/jev2.12110

• Gao, Jing et al. “Exosomes Promote Pre-Metastatic Niche Formation in Gastric Cancer.” Frontiers in oncology vol. 11652378. 24 May. 2021, doi:10.3389/fonc.2021.652378

• Prajapati, Sushant et al. “Biomimetic Nanotechnology: A Natural Path Forward for Tumor-Selective and Tumor-Specific NIR Activable Photonanomedicines.” Pharmaceutics vol. 13,6 786. 25 May. 2021, doi:10.3390/pharmaceutics13060786

• Sun, Ruiting et al. “Proteomic profiling of single extracellular vesicles reveals colocalization of SARS-CoV-2 with a CD81/integrin-rich EV subpopulation in sputum from COVID-19 severe patients.” Frontiers in immunology vol. 14 1052141. 12 May. 2023, doi:10.3389/fimmu.2023.1052141

• Kong, Lingnan et al. “Ascites-derived CDCP1+ extracellular vesicles subcluster as a novel biomarker and therapeutic target for ovarian cancer.” Frontiers in oncology vol. 13 1142755. 3 Jul. 2023, doi:10.3389/fonc.2023.1142755

• Zhai, Ranran et al. “Genetic and phenotypic links between obesity and extracellular vesicles.” Human molecular genetics vol. 31,21 (2022): 3643-3651. doi:10.1093/hmg/ddac069

• Min, Yuxin et al. “Single extracellular vesicle surface protein-based blood assay identifies potential biomarkers for detection and screening of five cancers.” Molecular oncology vol. 18,3 (2024): 743-761. doi:10.1002/1878-0261.13586

• Wen, Yi et al. “Cell-derived nanovesicles prepared by membrane extrusion are good substitutes for natural extracellular vesicles.” Extracellular vesicle vol. 1 (2022): 100004. doi:10.1016/j.vesic.2022.100004